QSRA is a quality-value guided de novo short read assembler. All high-throughput sequencing platforms incorporate errors in their output, so QSRA is a short-read assembler must be designed to account for these errors while still utilizing all available data.
QSRA v1.0 - 4/11/2009
Version 1.0 is now publicly available and has been published in BMC Bioinformatics. In test against other short-read assemblers:
- QSRA produced assemblies of the highest genomic coverage,
- QSRA is extremely competitive in its longest contig and N50/N80 contig lengths
- It's results are of a quality comparable to those of EDENA and VELVET
- A detailed analysis of QSRA compared to other short-read assemblers can be found in the paper here.
Version 0.5 marks the first developmental release of Supersplat that is available to the general scientific community.
- Given the fasta formatted reference file and the fast formatted HTS reads file, Supersplat will comprehensively search the reads for potential splice jucntions.
- Supersplat generates a large amount of output and can be intense on underpowered systems. Make sure that your system meets the recommended specs.